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| Registros recuperados : 26 | |
| 5. |  | ROCHA, R. B.; CRUZ, C. D.; BARROS, W. S.; FERREIRA, F. M.; ARAÚJO, E. F. de. Comparisons of segregating populations for genetic mapping. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 4, n. 4, p. 408-415, Dec. 2004.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | MURO-ABAD, J. I.; ROCHA, R. B.; CRUZ, C. D.; PIRES, I. E.; ARAÚJO, E. F. de. Crosses recommendation method for obtaining Eucalyptus spp. hybrids assisted by molecular markers. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 1, p. 71-79, Mar. 2005.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | ROCHA, R. B.; MURO-ABAD, J. I.; CRUZ, C. D.; BARROS, W. S.; BARROS, E. G.; ARAÚJO, E. F. de. Genetic mapping of oligogenic traits using maximum likelihood functions: backcrossing populations. Revista Brasileira de Biometria, São Paulo, v. 26, n. 3, p. 7-21, 2008. Meta 2008.| Biblioteca(s): Embrapa Rondônia. |
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| 13. | | AMARAL, F. H. C.; FURTINI NETO, A. E.; CURI, N.; INDA, A. V.; ARAÚJO, E. F. de. Produtividade de Eucalyptus grandis e sua relação com a cinética de liberação de macronutrientes. Scientia Forestalis, Piracicaba, v. 43, n. 108, p. 979-991, dez. 2015.| Biblioteca(s): Embrapa Florestas. |
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| 15. | | SANTANA, M. F.; SILVA, J. C. F.; BATISTA, A. D.; RIBEIRO, L. E.; SILVA, G. F. da; ARAÚJO, E. F. de; QUEIROZ, M. V. de. Abundance, distribution and potential impact of transposable elements in the genome of Mycosphaerella fijiensis. BMC Genomics, v. 13, n. 1, p. 1-11, Dec. 2012.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 16. | | TORRES, A. R.; CURSINO, L.; MURO-ABAD, J. I.; GOMES, E. A.; ARAÚJO, E. F. de; HUNGRIA, M.; CASSINI, S. T. A. Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 4, p. 852-856, Oct./Dec. 2009.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 17. | | TORRES, A. R.; CURSINO, L.; MURO-ABAD, J. I.; GOMES, E. A.; ARAÚJO, E. F. de; HUNGRIA, M.; CASSINI, S. T. A. Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 4, p. 852-856, Oct./Dec. 2009.| Biblioteca(s): Embrapa Soja. |
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| 19. | | ROCHA, R. B.; BARROS, W. S.; MURO-ABAD, J. I.; TOMAZ, R. S.; CRUZ, C. D.; BARROS, E. G. de; ARAÚJO, E. F. de. Método para mapeamento de locos controladores de características oligogênicas. Ciência Rural, v. 40, n. 2, 2010.| Biblioteca(s): Embrapa Rondônia. |
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| 20. | | ROSADO, T. B.; TOMAZ, R. S.; ROCHA, R. B.; ROSADO, A. M.; ALVES, A. A.; ARAÚJO, E. F. de; ALFENAS, A. C.; CRUZ, C. D. Detection and mapping of a lethal locus in a eucalyptus hybrid population. Pesquisa Agropecuária Brasileira, Brasília, v. 46, n. 9, p.1021-1028, set. 2011.| Biblioteca(s): Embrapa Rondônia; Embrapa Unidades Centrais. |
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| Registros recuperados : 26 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TEIXEIRA, J. A.; RIBEIRO, J. B.; GONÇALVES, D. B.; QUEIROZ, M. V. de; ARAÚJO, E. F. de. |
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Afiliação: |
JANAINA APARECIDA TEIXEIRA, UFV; JOAO BATISTA RIBEIRO, CNPGL; DANIEL BONOTO GONÇALVES, UFSJ; MARISA VIEIRA DE QUEIROZ, UFV; ELZA FERNANDES DE ARAÚJO, UFV. |
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Título: |
Overproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Applied Biochemistry and Biotechnology, v. 169, n. 6, p. 1965-1977, 2013. |
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DOI: |
https://doi.org/10.1007/s12010-013-0121-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Inactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector). This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum. The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production. |
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Palavras-Chave: |
Gene inactivation; Gpd promoter; Overproduction; Penicillium griseoroseum. |
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Thesaurus NAL: |
polygalacturonase. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02088naa a2200241 a 4500
001 1978792
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12010-013-0121-6$2DOI
100 1 $aTEIXEIRA, J. A.
245 $aOverproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene.$h[electronic resource]
260 $c2013
520 $aInactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector). This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum. The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production.
650 $apolygalacturonase
653 $aGene inactivation
653 $aGpd promoter
653 $aOverproduction
653 $aPenicillium griseoroseum
700 1 $aRIBEIRO, J. B.
700 1 $aGONÇALVES, D. B.
700 1 $aQUEIROZ, M. V. de
700 1 $aARAÚJO, E. F. de
773 $tApplied Biochemistry and Biotechnology$gv. 169, n. 6, p. 1965-1977, 2013.
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